《植物生理学报》 2018, 54(4): 677-685

澳洲鸽子石斛组织培养快速繁殖研究

莫远琪^1,2, 郑枫¹, 房林¹, 李琳¹, 江南^3,*, 吴坤林^1,*, 曾宋君^1,4,*

¹中国科学院华南植物园华南农业植物分子分析与遗传改良重点实验室, 广州510650; ²中国科学院大学, 北京100049; ³东莞农业科学研究中心, 广东东莞523000; ⁴中国科学院华南植物园广东省应用植物学重点实验室, 广州510650

通信作者：江南；E-mail: dgjn2@126.com, wkl8@scib.ac.cn, zengsongjun@scib.ac.cn

摘　要：

以澳洲鸽子石斛(Dendrobium kingianum Bidwill)幼嫩假鳞茎为外植体进行组织培养和快速繁殖研究。结果表明: 采用合适的消毒方式, 外植体消毒成功率可达92.5%。MS培养基添加5.0 mg·L^-1 6-BA适合用于不定芽的诱导和前期增殖, MS培养基+0.5 mg·L^-1 NAA+2.0 mg·L^-1 KT的增殖倍数最高, 为6.96。丛生芽诱导出愈伤组织的最佳培养基为MS+0.25 mg·L^-1 2,4-D, 诱导率为80%; 愈伤组织分化的最佳培养为MS+1.0 mg·L^-1 6-BA, 愈伤组织分化芽数为381.99个·g^-1。MS+2.0 mg·L^-1 IBA+10%香蕉泥+1 g·L^-1活性炭最适合于生根壮苗培养; 试管苗在兰石:泥碳土(1:1)的混合基质中移栽3个月后, 成活率达100%, 且生长速度快, 基质成本较低, 适合澳洲鸽子石斛试管苗的商业化栽培。

关键词：澳洲鸽子石斛; 假鳞茎; 愈伤组织; 增殖; 壮苗; 移栽

收稿：2018-01-05   修定：2018-04-08

资助：国家重点研发计划项目(2016YFC0503104)、东莞市社会科技发展项目(2016108101001)、中国科学院战略生物资源服务网络计划植物种质资源创新项目(ZSZC-008)和广东省现代农业产业技术体系花卉创新团队项目(2016LM1142)。

Tissue culture and rapid propagation of Dendrobium kingianum Bidwill

MO Yuan-Qi^1,2, ZHENG Feng¹, FANG Lin¹, LI Lin¹, JIANG Nan^3,*, WU Kun-Lin^1,*, ZENG Song-Jun^1,4,*

¹Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; ²University of Chinese Academy of Sciences, Beijing 100049, China; ³Dongguan Agricultural Science Research Center, Dongguan, Guangdong 523000, China; ⁴Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

Corresponding author: JIANG Nan; E-mail: dgjn2@126.com, wkl8@scib.ac.cn, zengsongjun@scib.ac.cn

Abstract:

The tissue culture and rapid propagation system of Dendrobium kingianum Bidwill was established by using the pseudobulbs as explants. The results showed that the success rate of explants disinfection reached 92.5% with proper disinfection methods. MS supplemented with 5 mg·L^-1 6-BA was suitable for adventitious bud induction and proliferation for previous generations. The most suitable medium for the multiplication was 0.5 mg·L^-1 NAA and 2.0 mg·L^-1 KT and the multiplication rate was 6.96. MS medium supplemented with 0.25 mg·L^-1 2,4-D was the most appropriate for callus induction and the callus induction rate was 80%. MS with 1.0 mg·L^-1 6-BA was most suitable for callus differentiation and 381.88 buds adventitious buds were induced for 1.0 g callus. MS supplemented with 2.0 mg·L^-1 IBA, 1 g·L^-1 activated carbon and 10% banana homogenate was suitable for the rooting and growth of cluster buds. On the mixture substrate of orchid stone: peat soil=1:1 (V:V), the cultured plantlets grew fast and the survival rate was 100%, which suggested that the substrate was suitable for transplanting in the commercial production.

Key words: Dendrobium kingianum; pseudobulbs; callus; protocorm-like-body; proliferation; growth culture; transplantion